Google scholar 上的Karroten

Molecular characterization, expression, polymorphism of NR5A2 and its relationship with litter size in Hu sheep

YX Li, J Zhang, Y Qian, CH Meng… - Genetics and …, 2015 - funpecrp.com.br

… Amplification products were separated and visualized by agarose gel electrophoresis, and purifiedwith a DNA gel extraction kit (Karroten, Nanjing, China). Recovered DNA fragments were ligatedinto pMD18-T vectors and transformed into Escherichia coli DH5α …

 

Molecular Characterization and Expression of PFT, an FHB Resistance Gene at the Fhb1 QTL in Wheat

Y He, X Zhang, Y Zhang, D Ahmad, L Wu… - …, 2018 - Am Phytopath Society

… Genomic DNA was extracted from the fresh leaves of 348 wheat accessions by using Karroten Plant Genomic DNA Extraction Kit (Karroten). The RNA and DNA quality and quantity weredetermined by agarose gel electrophoresis and NanoDrop 2000 (Thermo Scientific) …

 

Mitochondrial genome is paternally inherited in Cucumis  allotetraploid (C.× hytivus) derived by interspecific hybridization

J Shen, MG Kere, JF Chen - Scientia Horticulturae, 2013 - Elsevier

… after staining with ethidium bromide. 2.3. Cloning and sequences analysis. The PCR
products of the expected size were recovered and purified using a DNA Gel Extraction
Kit (Karroten, China). The purified fragments were cloned …

 

Genetic diversity of Ty1-copia retrotransposons in a wild species of Cucumis (C. hystrix)

B Jiang, ZM Wu, QF Lou, D Wang, WP Zhang… - Scientia horticulturae, 2010 - Elsevier

… 2.3. Cloning and sequencing. The PCR products of expected size were recovered and purifiedby DNA Gel Extraction Kit (Karroten, China). The purified fragments were cloned into the vectorpMD19-T (Takara, Japan) and then transformed into E. coli strain DH5α …

 

Retrotransposon- and microsatellite sequence-associated genomic changes in early generations of a newly synthesized allotetraploid Cucumis × hytivus Chen & …

B Jiang, Q Lou, Z Wu, W Zhang, D Wang… - Plant molecular …, 2011 - Springer

… DNA fragments with altered patterns between parental lines and the allotetraploid (parental bandsabsent in the allotetraploid or novel bands in the allotetraploid) were excised from the gel, purifiedwith a DNA gel extraction kit (Karroten, China), cloned into the pMD19-T vector …

 

 Diversity of endophytic fungi of Myricaria laxiflora grown under pre-and post-flooding conditions

W Tian, YH Bi, W Zeng, W Jiang, YH Xue… - Genet Mol …, 2015 - geneticsmr.com

… Following morphologic observation and microscopic examination, ITS sequence analysis wascarried out on each isolate. Total genomic DNA was extracted from cultures at 7-14 days, usinga microbe genomic DNA isolation kit (Karroten Life Scientific, China) …

Transcription profiles of genes related to hormonal regulations under salt stress in sweet sorghum

Z Yang, Y Wang, X Wei, X Zhao, B Wang… - Plant Molecular Biology …, 2017 - Springer

… Total RNA was isolated from the leaves and seminal roots of each genotype treated with 0 and150 mM NaCl for 48 h. Total RNA isolation was performed using a Total Plant RNA Extraction Kit (Karroten, Beijing, China) following the manufacturer's protocols …

 

Transcriptome analysis of sweet Sorghum inbred lines differing in salt tolerance provides novel insights into salt exclusion by roots

Z Yang, H Zheng, X Wei, J Song, B Wang, N Sui - Plant and Soil, 2018 - Springer

… Total RNA extraction Total RNA was isolated from the primary roots of each genotype treated with 0 and 150 mM NaCl for 48 h using a Total Plant RNA Extraction Kit (Karroten, Bei- jing, China) following the manufacturer's protocols as described previously (Sui et al. 2015) …

 

Ultra performance liquid chromatography/ quadrupole-time -of-flight mass spectrometry for determination of avicularin metabolites produced by a human intestinal …

M Zhao, J Xu, D Qian, J Guo, S Jiang, E Shang… - …Journal of Chromatography B, 2014 - Elsevier… 2.7. 16S rRNA gene sequencing and phylogenetic analysis. The genomic DNA of strain 46 wasextracted from the isolate using a Karroten Genomic DNA Purification kit (Karroten, China) … ThePCR product was purified from the agarose gel using a Karroten gel purification kit …

 

Application of ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry to determine the metabolites of orientin produced by …

J Xu, D Qian, S Jiang, J Guo, E Shang, J Duan… - … of Chromatography B, 2014 - Elsevier

… 2.7. 16S rRNA gene sequencing and phylogenetic analysis. Genomic DNA was extracted fromthe isolate using a Karroten genomic DNA purification kit (Karroten, China) … The PCR product was purified from the agarose gel using a Karroten gel purification kit …

 

 

The transcriptome of NaCl-treated Limonium bicolor leaves reveals the genes controlling salt secretion of salt gland

F Yuan, MJA Lyu, BY Leng, XG Zhu, BS Wang - Plant molecular biology, 2016 - Springer

… sequencing analysis. The total RNA was extracted using a Total Plant RNA Extraction
Kit (Karroten, Beijing, China) and purified with an RNeasy Mini RNA kit (Qiagen,
Germany) including a one-column DNase digestion. The …

 

Comparative transcriptome analysis of developmental stages of the Limonium bicolor leaf generates insights into salt gland differentiation

F Yuan, MJUAMY LYU, BY LENG… - Plant, cell & …, 2015 - Wiley Online Library

… Total RNA from different samples was extracted using a Total Plant RNA Extraction
kit (Karroten, Beijing, China) and purified using the RNAeasy Mini RNA kit (Qiagen,
Shanghai, China) including on-column DNase digestion …

 

LTR retrotransposons cause expression changes of adjacent genes in early generations of the newly formed allotetraploid Cucumis hytivus

L Jia, Q Lou, B Jiang, D Wang, J Chen - Scientia Horticulturae, 2014 - Elsevier

… ethidium bromide. The expected fragments were recovered by DNA Gel Extraction
Kit (Karroten, China) and then ligated into the pMD19-T vector (TaKaRa, Japan) and
transformed into Escherichia coli strain DH5α. Recombinant …

 

Physiological and molecular evidence for Na+ and Cl− exclusion in the roots of two Suaeda salsa populations

Q Liu, R Liu, Y Ma, J Song - Aquatic Botany, 2018 - Elsevier

… Total RNA was extracted from roots of S. salsa from the intertidal zone and inland saline soils(a 0.2 g subsample per biological replicate) using a Total Plant RNA Extraction Kit (Karroten,Beijing, China) following the manufacturer's protocols (Sui et al., 2015) …

 

 

The interactions between gut microbiota and entomopathogenic fungi: a potential approach for biological control of Blattella germanica (L.)

F Zhang, XX Sun, XC Zhang, S Zhang… - Pest management …, 2018 - Wiley Online Library

… 2.8 PCR amplification and pyrosequencing. Gut homogenates from 60 cockroaches
were prepared and DNA was extracted using the K2306 Karroten Microbial Genomic
DNA extraction kit (Novoprotein Scientific Inc., Shanghai, China) …

 

NADP-malate dehydrogenase of sweet sorghum improves salt tolerance of Arabidopsis thaliana

Y Guo, Y Song, H Zheng, Y Zhang… - Journal of agricultural …, 2018 - ACS Publications

… 127 Cloning the sequence of SbNADP-ME 128 Total RNA was isolated from leaves of sweetsorghum using the Total Plant RNA Extraction Kit 129 (Karroten 1103) in accordance withthe manufacturer's instructions. The full-length NADP-ME gene 130 …

 

Transcriptome profiling of genes involved in photosynthesis in Elaeagnus angustifolia L. under salt stress

J Lin, JP Li, F Yuan, Z Yang, BS Wang, M Chen - Photosynthetica - Springer

… RNA-Seq analysis: Total RNA was extracted from the leaves of E. angustifolia seedlings
treated with 0 or 150 mM NaCl for 48 h using the Total Plant RNA Extraction Kit (Karroten,
Beijing, China) following the manufacturer's protocol …

 

 

[HTML] Rosiglitazone Exerts an Anti-depressive Effect in Unpredictable Chronic Mild-Stress-Induced Depressive Mice by Maintaining Essential Neuron Autophagy …

Z Zhao, L Zhang, XD Guo, LL Cao, TF Xue… - Frontiers in molecular …, 2017 - frontiersin.org

… Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR). Total RNA wasisolated from brain tissues, cell samples, or cultured cells using TRIzol reagent (Karroten) andprepared for quantitative reverse transcriptase PCR using Master Mix (TaKaRa, Japan) …

 

[HTML] Overexpression of glycerol-3-phosphate acyltransferase from Suaeda salsa improves salt tolerance in Arabidopsis

N Sui, S Tian, W Wang, M Wang, H Fan - Frontiers in plant science, 2017 - frontiersin.org

… determined 14 days later. Cloning and Sequencing of SsGPAT. Total RNA was isolated
from the leaves of S. salsa using a Total Plant RNA Extraction Kit (Karroten 1103)
according to the manufacturer's instructions. In the experiment …

 

A system for the transformation and regeneration of the recretohalophyte Limonium bicolor

F Yuan, M Chen, J Yang, B Leng, B Wang - In Vitro Cellular & …, 2014 - Springer

… Total RNA from the uninfected and infected regenerated plantlets was extracted using the TotalPlant RNA Extraction Kit (Karroten, Beijing, China) and then first-strand complementary DNAs(cDNAs) were synthesized from DNaseI-treated total RNA using ReverTra Ace qPCR …

 

Activation of ATP-sensitive potassium channel by iptakalim normalizes stress-induced HPA axis disorder and depressive behaviour by alleviating inflammation and …

XJ Zhao, Z Zhao, DD Yang, LL Cao, L Zhang, J Ji… - Brain research …, 2017 - Elsevier

… qRT-PCR). The hypothalamus was separated from the frozen brain. Total RNA was
extracted by using a TRIzol reagent (Karroten) and prepared for quantitative reverse
transcriptase PCR by using Master Mix (TaKaRa, Japan) …

 

Gene-indexed mutations in maize

X Lu, J Liu, W Ren, Q Yang, Z Chai, R Chen, L Wang… - Molecular plant, 2018 - Elsevier

… plants. Construction of Exome Sequencing Libraries. Genomic DNA was isolated
from young leaves of 1090 mutated plants in the M 1 generation using the Plant
Genomic DNA Extraction Kit (Karroten, Nanjing, Jiangsu, China) …

 

 [PDF] NaCl, 蔗糖和KH2PO4 对红叶桃离体叶片花色苷合成因表达的影响

文杨， 韩键， 姜卫兵， 王小青， 房经贵， 张斌斌， 马瑞娟 - 园艺学报, 2016 - ahs.ac.cn

… 按照植物总RNA 提取试剂盒Karroten产品说明书进行桃叶片RNA
的提取，并用TaKaRa Recombinant DNase I（RNase-free）去除RNA 中的DNA。提取的RNA 立即进行反转录，或保存在–80 ℃冰箱备用。 以提取的总RNA 为模板，利用TaKaRa Prime ScriptTM …

 

[PDF] 基于基因组重测序信息构建玉米第5 染色体的片段代换系

林峰， 葛敏， 宝华宾， 赵涵 - 玉米科学, 2015 - ymkx.com.cn

… 将群体所有单株及亲本取样，植物叶片用低温 真空干燥仪干燥后，采用Karroten 植物基因组DNA提取试剂盒抽提DNA，具体步骤详见试剂盒说明 书。DNA加适量TE溶解后，4℃或-20℃贮藏待用。PCR反应体系为25 μL，包含5 pmol两侧引物，2.5 μL 10 x buffer，1.25 nmol dNTP，1 U …

 

[PDF] 海滨雀稗体细胞突变体SP2008-3 的特异性分析

常盼盼， 钟小仙， 刘智微 - 草业学报, 2012 - cyxb.magtech.com.cn

… 拍照和统计染色体数目。 １．２．４ ＲＡＰＤ分子鉴定取海滨雀稗体细胞突变体ＳＰ２００８３、原始对照Ａｄａｌａｙｄ（ＣＫ１）及其幼穗离体培养再 生植株（ＣＫ２）的幼嫩叶片，采用Karroten公司生产的植物ＤＮＡ提取试剂盒（Karroten Plant Genomic DNA …

 

[HTML] 利用Insertion/Deletion (InDel) 分子标记检测玉米互交种混杂的原理及应用

葛敏， 蒋璐， 张晓林， 赵涵， 张体付 - 《 分子植物育种》 官方网站, 2012 - biopublisher.cn

… 也适用于具有可用InDel标记的其它作物互交种的鉴定。 3材料与方法 3.1材料 供试材料为玉米自交系B73、Mo17、正交种B73×Mo17(BM)及反交种Mo17×B73(MB)。 3.2 DNA提取 B73、Mo17、BM、MB叶片DNA以及5个BM、5个MB胚乳样本DNA提取参照Karroten DNA提取试剂盒，DNA …

 

[PDF] 紫花苜蓿γ-生育酚甲基转移酶(γ-TMT) 基因的克隆与逆境下的表达分析

贾会丽， 王学敏， 高洪文， 董洁， 王运琦， 刘建宁… - 草业学报, 2012 - cyxb.lzu.edu.cn

… 拍照和统计染色体数目。 １．２．４ ＲＡＰＤ分子鉴定取海滨雀稗体细胞突变体ＳＰ２００８３、原始对照Ａｄａｌａｙｄ（ＣＫ１）及其幼穗离体培养再 生植株（ＣＫ２）的幼嫩叶片，采用Karroten公司生产的植物ＤＮＡ提取试剂盒（Karroten Plant Genomic DNA  …